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Answer questions regarding the physical and chemical properties of dna being utilized.
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Task 1: Use the website (https://learn.genetics.utah.edu/content/labs/gel/) to visualize this technique. Answer questions regarding the physical and chemical properties of DNA being utilized.
What is caused the DNA to be “moved” through the gel?
Which size strands move the fastest? Why?
What is the purpose of the DNA Size Standard? What is the consequence of forgetting to include this?Task 2: Complete the interactive activity (https://learn.genetics.utah.edu/content/labs/pcr/), answering the questions below:
What are primers? What role do they serve?
Why do nucleotides need to be added to the reaction?
What is the purpose of DNA Polymerase? What would happen to the reaction if you forgot to add this?Task 3: Complete Case One by selecting Launch Interactive (https://www.biointeractive.org/classroom-resources/csi-wildlife) and answering the following questions. This includes: The Crime Scene, How DNA Profiling works, Finding a Match, Case Solved.
When you added the four Markers (under Application) you’ll notice that they are not identical to the two elephants. Explain why this is, even though they are the type of animal.
If you were to only include Marker FH19 and Marker FH127 in this analysis, would you come to a different conclusion for the first “Finding a Match” sample examined? Explain.